IgG anti-IgE autoantibodies in visceral leishmaniasis
Mem. Inst. Oswaldo Cruz
;
97(1): 101-103, Jan. 2002. ilus, graf
Artículo
en Inglés
| LILACS
| ID: lil-306074
ABSTRACT
Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa epsilon-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Autoanticuerpos
/
Inmunoglobulina E
/
Inmunoglobulina G
/
Anticuerpos Antiidiotipos
/
Leishmaniasis Visceral
Límite:
Humanos
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2002
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Hospital Universitário Prof. Edgar Santos/BR
/
Universidade Federal da Bahia/BR
Similares
MEDLINE
...
LILACS
LIS