Experimental infection and horizontal transmission of Bartonella henselae in domestic cats
Rev. Inst. Med. Trop. Säo Paulo
;
43(5): 257-261, Sept.-Oct. 2001. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-307998
RESUMO
In order to study B. henselae transmission among cats, five young cats were kept in confinement for two years, one of them being inoculated by SC route with B. henselae (10(5) UFC). Only occasional contact among cats occurred but the presence of fleas was observed in all animals throughout the period. Blood culture for isolation of bacteria, PCR-HSP and FTSZ (gender specific), and BH-PCR (species-specific), as well as indirect immunofluorescence method for anti-B. henselae antibodies were performed to confirm the infection of the inoculated cat as well as the other naive cats. Considering the inoculated animal, B. henselae was first isolated by blood culture two months after inoculation, bacteremia last for four months, the specific antibodies being detected by IFI during the entire period. All contacting animals presented with bacteremia 6 months after experimental inoculation but IFI did not detect seroconversion in these animals. All the isolates from these cats were characterized as Bartonella (HSP and FTSZ-PCR), henselae (BH-PCR). However, DNA of B. henselae could not be amplified directly from peripheral blood by the PCR protocols used. Isolation of bacteria by blood culture was the most efficient method to diagnose infection compared to PCR or IFI. The role of fleas in the epidemiology of B. henselae infection in cats is discussed
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Infecciones por Bartonella
/
Enfermedades de los Gatos
/
Bartonella henselae
/
Anticuerpos Antibacterianos
Tipo de estudio:
Estudio diagnóstico
/
Guía de Práctica Clínica
Límite:
Animales
Idioma:
Inglés
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Asunto de la revista:
Medicina Tropical
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade de Säo Paulo/BR
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