Acid phosphatase activity in ungerminated conidia from Colletotrichum graminicola as determined by spectrophotometric and cytochemical methods
Braz. j. morphol. sci
;
17(2): 81-85, jul.-dez. 2000. ilus
Artículo
en Inglés
| LILACS
| ID: lil-313962
RESUMO
The acid phosphatase in ungerminated conidia from Colletotrichum graminicola, a corn pathogen, was investigated using spectrophotometric and cytochemical methods. Acid phosphatase activity was studied in a homogenate obtained by fragmentation of ungerminated conidia. With p-nitrophenylphosphate as substrate, the apparent Vmax and Km were 1.000 nmol p-nitrophenol/mg of protein/min and 0.631 mM, respectively. The pH and temperature optima were 5.5 and 60 graus Celsius, respectively. A cytochemical ultrastructural assay showed deposition of the reaction product inside vacuoles but not extracellularly on the cell surface. The permeabilization of conidia with Triton X-100 increased acid phosphatase activity eight fold. Compared to other procedures, our method was fast, easy to perform and gave consistent results.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Fosfatasa Ácida
/
Vacuolas
Idioma:
Inglés
Revista:
Braz. j. morphol. sci
Asunto de la revista:
Anatomía
Año:
2000
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Federal University of Paraná/BR
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