Cytotoxic and Antiviral Activities of Colombian Medicinal Plant Extracts of the Euphorbia genus
Mem. Inst. Oswaldo Cruz
;
97(4): 541-546, June 2002. tab
Artículo
en Inglés
| LILACS
| ID: lil-314510
ABSTRACT
Forty-seven plant extracts of 10 species of the genus Euphorbia (Euphorbiaceae) used by Colombian traditional healers for the treatment of ulcers, cancers, tumors, warts, and other diseases, were tested in vitro for their potential antitumour (antiproliferative and cytotoxic) and antiherpetic activity. To evaluate the capacity of the extracts to inhibit the lytic activity of herpes simplex virus type 2 (HSV-2) and the reduction of viability of infected or uninfected cell cultures, the end-point titration technique (EPTT) and the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay were used, respectively. The therapeutic index of the positive extracts for the antiviral activity was determined by calculating the ratio CC50 (50 percent cytotoxic concentration) over IC50 (50 percent inhibitory concentration of the viral effect). Five of the 47 extracts (11 percent) representing 3 out of 10 Euphorbia species (30 percent) exhibited antiherpetic action; the highest activity was found in the leaf/stem water-methanol extracts from E. cotinifolia and E. tirucalli. The therapeutic indexes of these two plant species were > 7.1; these extracts exhibited no cytotoxicity. Six extracts (13 percent) representing 4 plant species (40 percent) showed cytotoxic activity. The highest cytotoxicity was found in the dichloromethane extract obtained from E. cotinifolia leaves and the CC50 values for the most susceptible cell lines, HEp-2 and CHO, were 35.1 and 18.1 æg/ml, respectively
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Antivirales
/
Extractos Vegetales
/
Herpesvirus Humano 2
/
Euphorbia
Límite:
Animales
/
Femenino
/
Humanos
País/Región como asunto:
America del Sur
/
Colombia
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2002
Tipo del documento:
Artículo
País de afiliación:
Colombia
Institución/País de afiliación:
Grupo GIEM/CO
/
Grupo Inmunovirología-Biogénesis/CO
/
Universidad de Antioquia/CO
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