Diffuse and enteroaggregative patterns of adherence of escherichia coli isolated from stools of children in northeastern Brazil
Braz. j. microbiol
;
32(4): 313-319, Oct.-Dec. 2001. tab
Artículo
en Inglés
| LILACS
| ID: lil-314805
ABSTRACT
Childhood diarrheal diseases remain highly endemic in northeastern Brazil. The attributable fraction of all diarrheal diseases among children less than 2 years of age due to Escherichia coli was examined in a 2 year prospective study in two large urban centers of Brazil. Between May 1997 and June 1999, fecal E.coli isolates from 237 children with diarrhea (217 acute and 20 persistent cases) and 231 children without diarrhea (controls) attending two hospitals in Northeast Brazil were tested for their pattern of adherence to HEp-2 cells and for colony hybridization with DNA probes specific for the six pathotypes of diarrheagenic E.coli. Enteroinvasive E.coli, enterotoxigenic E.coli and enterohemorrhagic E.coli were not isolated from any children. Diffusely adherent E.coli (DAEC) and enteroaggregative E.coli (EAEC) were the most frequent isolates with similar frequencies from children with or without diarrhea. Atypical EPEC (EAF-negative) strains were isolated with similar frequency from both cases 5.5 per cente and controls 5.6 per cente. Enteropathogenic E.coli (typical EPEC) strains, characterized by localized adherence pattern of adherence, hybridization with the EAF probe, and belonging to the classical O serogroups, were significantly associated with diarrhea (P=0.03). These E.coli strains associated with diarrhea accounted for 9 per cente of all children with diarrhea. Collectively, in Northeast Brazil, E.coli strains comprise a small proportion of severe diarrhea prevalence in children.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Técnicas In Vitro
/
Adhesinas de Escherichia coli
/
Diarrea Infantil
/
Escherichia coli
Tipo de estudio:
Estudio observacional
/
Factores de riesgo
Límite:
Niño
/
Humanos
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Hospital Universitário Materno-Infantil/BR
/
Universidade Federal de São Paulo/BR
/
Universidade Federal do Rio Grande do Norte/BR
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