Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de shigella flexneri aisladas en niños chilenos con diarrea aguda / Molecular characterization of resistance mechanisms to chloramphenicol in shigella flexneri strains isolated from chilean children with acute diarrhea
Rev. méd. Chile
;
130(3): 275-280, mar. 2002. ilus, tab
Artículo
en Español
| LILACS
| ID: lil-314853
ABSTRACT
Background:
Chloramphenicol is one of the therapeutic options for shigellosis, but resistance to this antimicrobial is increasing.Aim:
To characterize molecular mechanisms conferring resistance to chloramphenicol (CmR) in Shigella flexneri strains isolated from Chilean children with acute diarrhea. Material andmethods:
Thirty one Shigella filexneri strains, including 22 with the CmR phenotype were analyzed. Strains were tested for antimicrobial susceptibility by plate dilution and for the presence of an internal fragment of the cat gene encoding for chloramphenicol o-acetyl-transferase, by polymerase chain reaction and Southern blot analysis.Results:
All CmR strains had a minimal inhibitory concentration over 64 µg/ml and amplified the internal fragment of the cat gene. Southern blot analyses indicated that this gene was located in the bacterial chromosome.Conclusions:
Resistance to chloramphenicol in this group of Shigella flexneri strains was mediated by a chromosomally located cat gene
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Shigella flexneri
/
Resistencia al Cloranfenicol
/
Diarrea
Límite:
Niño
/
Humanos
País/Región como asunto:
America del Sur
/
Chile
Idioma:
Español
Revista:
Rev. méd. Chile
Asunto de la revista:
Medicina
Año:
2002
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Chile
Institución/País de afiliación:
Universidad de Chile/CL
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