Correlações entre o teor de colágeno no miocárdio ventricular esquerdo humano medido através de métodos / Correlations between the collagen content of the human left ventricular myocardium, measured by biochemical and morphometric methods
Arq. bras. cardiol
;
79(1): 10-19, July 2002. tab, graf
Artículo
en Portugués, Inglés
| LILACS
| ID: lil-316162
ABSTRACT
OBJECTIVE:
To assess, in myocardium specimens obtained from necropsies, the correlation between the concentration of hydroxyproline, measured with the photocolorimetric method, and the intensity of fibrosis, determined with the morphometric method.METHODS:
Left ventricle myocardium samples were obtained from 45 patients who had undergone necropsy, some of them with a variety of cardiopathies and others without any heart disease. The concentrations of hydroxyproline were determined with the photocolorimetric method. In the histologic sections from each heart, the myocardial fibrosis was quantified by using a light microscope with an integrating ocular lens.RESULTS:
A median of, respectively, 4.5 and 4.3 mug of hydroxyproline/mg of dry weight was found in fixed and nonfixed left ventricle myocardium fragments. A positive correlation occurred between the hydroxyproline concentrations and the intensity of fibrosis, both in the fixed (Sr=+0.25; p=0.099) and in the nonfixed (Sr=+0.32; p=0.03) specimens.CONCLUSION:
The biochemical methodology was proven to be adequate, and manual morphometry was shown to have limitations that may interfere with the statistical significance of correlations for the estimate of fibrosis intensity in the human myocardium
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Colágeno
/
Ventrículos Cardíacos
/
Hidroxiprolina
Límite:
Adolescente
/
Adulto
/
Aged80
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
/
Portugués
Revista:
Arq. bras. cardiol
Asunto de la revista:
Cardiología
Año:
2002
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Faculdade de Medicina do Triângulo Mineiro/BR
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