Experimental model of Candida Albicans (serotypes A and B) adherence In Vitro
Braz. j. microbiol
;
32(3): 163-169, July-Sept., 2001. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-316963
RESUMO
The adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37§C and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Levaduras
/
Técnicas In Vitro
/
Candida albicans
/
Candidiasis
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Instituto Adolfo Lutz/BR
/
Universidade de Säo Paulo/BR
/
Universidade de Säo Paulo, Säo Paulo/BR
/
Universidade de Säo Paulo, Säo Paulo, SP, Brasil/BR
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