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Studies on the efficiency of measles virus antigen production using VERO cell culture in a microcarrier system
Mendonça, R. Z; Vaz-de-Lima, L. R. A; Oliveira, M. I; Pereira, C. A; Hoshino-Shimizu, S.
  • Mendonça, R. Z; Insituto Butantan. Laboratorio de Imunologia Viral. São Paulo. BR
  • Vaz-de-Lima, L. R. A; Instituto Adolfo Lutz. Divisão de Biologia Médica. Secao de Imunologia. São Paulo. BR
  • Oliveira, M. I; Instituto Adolfo Lutz. Divisão de Biologia Médica. Seção de Imunologia. São Paulo. BR
  • Pereira, C. A; Instituto Butantan. Laboratorio de Imunologia Viral. São Paulo. BR
  • Hoshino-Shimizu, S; Instituto Adolfo Lutz. Divisão de Biologia Médica. Seção de Imunologia. São Paulo. BR
Braz. j. med. biol. res ; 27(7): 1575-1587, Jul. 1994.
Artículo en Inglés | LILACS | ID: lil-319788
RESUMO
1. A large amount of antigen is required to conduct seroepidemiologic surveys of measles. Thus, a process to obtain measles virus antigen using a bioreactor was standardized. 2. The virus was grown in a 3.7-1 culture of VERO cells using a Celligen cell culture system containing 2 mg/ml of microcarriers (cytodex I) at 37 degrees C and 60 rpm. The cultures infected with 0.5 m.o.i. of measles virus were harvested after the appearance of the cytopathic effect. The virus suspension was clarified and concentrated by ultracentrifugation. Intracellular and extracellular virus titers were determined by hemagglutination (HA) and by induction of a cytopathic effect in cell culture (TCID50). 3. Intracellular virus presented 5-7 x 10(6) TCID50/0.1 ml, HA activity per 50 microliters equal to 32, with a total HA activity of 4,480 HA units (HAU) and specific activity of 116 HAU/mg. In the concentrated supernatants, the HA titer of extracellular virus was 64, with a total HA activity of 1,024 HAU and a specific activity of 1,600 HAU/mg. 4. The antigen obtained was suitable for the detection of antibodies against measles virus in assays such as ELISA and DOT-ELISA (using 1 micrograms/well to ELISA and 2 micrograms/DOT). 5. The microcarrier system produced antigen sufficient for 26 ELISAs/ml compared to 5.7 ELISAs/ml obtained for the static culture system.
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Índice: LILACS (Américas) Asunto principal: Virus del Sarampión / Antígenos Virales Límite: Animales / Humanos Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 1994 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Insituto Butantan/BR / Instituto Adolfo Lutz/BR / Instituto Butantan/BR

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Índice: LILACS (Américas) Asunto principal: Virus del Sarampión / Antígenos Virales Límite: Animales / Humanos Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 1994 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Insituto Butantan/BR / Instituto Adolfo Lutz/BR / Instituto Butantan/BR