DNA polymorphism of schistosomes and their snail hosts
Mem. Inst. Oswaldo Cruz
;
90(2): 211-213, Mar.-Apr. 1995.
Artículo
en Inglés
| LILACS
| ID: lil-319900
ABSTRACT
Analysis of the genomes of schistosomes and one of their intermediate hosts, Biomphalaria glabrata, using Random Amplified Polymorphic DNA (RAPD) demonstrated that intraspecific genetic polymorphism in the parasite is limited but in the snail is highly pronounced. This suggests an important role for the snail in the determination of the epidemiology of the disease. In addition to their intraspecific stability, schistosome derived RAPDs exhibit a high level of interspecific polymorphism and are thus ideal for the construction of phylogenetic trees. For the detection of intraspecific polymorphisms extensive variation in the mitochondrial DNA is being exploited for the development of a PCR based test for Schistosoma mansoni. Gene level polymorphisms are being analyzed by Low Stringency Single Specific Primer PCR.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Polimorfismo Genético
/
Schistosoma
/
Biomphalaria
/
ADN
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
1995
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Centro de Pesquisas René Rachou-FIOCRUZ/BR
/
Universidade Federal de Minas Gerais/BR
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