Molecular aspects of Schistosoma mansoni female maturation
Mem. Inst. Oswaldo Cruz
; 90(2): 179-184, Mar.-Apr. 1995.
Article
en En
| LILACS
| ID: lil-319903
Biblioteca responsable:
BR1.1
ABSTRACT
Incubation of total protein extracts of Schistosoma mansoni with 3H 17-beta-estradiol and 20-hydroxyecdysone, revealed steroid binding proteins in both, male and female worms. The interaction of nuclear proteins with restriction fragments of the gender and stage-specific gene F-10 was investigated using the "Band-Shift" technique. Distinct male and female nuclear proteins bound to the fragments of this gene. Among the nuclear proteins, only those rich in cysteine residues bound to DNA. In vitro incubation of live worms with the estrogen antagonist Tamoxifen, altered the pattern of the DNA binding proteins, producing in females, a band profile similar to that obtained with male worm protein extracts. When Tamoxifen was injected into schistosome infected mice, the eggs produced by females presented an abnormal morphology, compatible with non-viable eggs. These results suggest that the regulation of transcription of the F-10 gene might involve steroid receptors.
Texto completo:
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Índice:
LILACS
Asunto principal:
Schistosoma mansoni
/
Proteínas del Huevo
/
Proteínas del Helminto
/
Proteínas de Unión al ADN
Límite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Año:
1995
Tipo del documento:
Article