Your browser doesn't support javascript.
loading
Molecular aspects of Schistosoma mansoni female maturation
Giannini, Ana Lúcia; Linhares, Sérgio Vasconcelos; Caride, Elena Cristina; Braga, Vânia Maria; Rumjanek, Franklin David.
Afiliación
  • Giannini, Ana Lúcia; Universidade Federal do Rio de Janeiro. ICB/CSS. Departamento de Bioquímica Médica. BR
  • Linhares, Sérgio Vasconcelos; Universidade Federal do Rio de Janeiro. ICB/CSS. Departamento de Bioquímica Médica. BR
  • Caride, Elena Cristina; Universidade Federal do Rio de Janeiro. ICB/CSS. Departamento de Bioquímica Médica. BR
  • Braga, Vânia Maria; Universidade Federal do Rio de Janeiro. ICB/CSS. Departamento de Bioquímica Médica. BR
  • Rumjanek, Franklin David; Universidade Federal do Rio de Janeiro. ICB/CSS. Departamento de Bioquímica Médica. BR
Mem. Inst. Oswaldo Cruz ; 90(2): 179-184, Mar.-Apr. 1995.
Article en En | LILACS | ID: lil-319903
Biblioteca responsable: BR1.1
ABSTRACT
Incubation of total protein extracts of Schistosoma mansoni with 3H 17-beta-estradiol and 20-hydroxyecdysone, revealed steroid binding proteins in both, male and female worms. The interaction of nuclear proteins with restriction fragments of the gender and stage-specific gene F-10 was investigated using the "Band-Shift" technique. Distinct male and female nuclear proteins bound to the fragments of this gene. Among the nuclear proteins, only those rich in cysteine residues bound to DNA. In vitro incubation of live worms with the estrogen antagonist Tamoxifen, altered the pattern of the DNA binding proteins, producing in females, a band profile similar to that obtained with male worm protein extracts. When Tamoxifen was injected into schistosome infected mice, the eggs produced by females presented an abnormal morphology, compatible with non-viable eggs. These results suggest that the regulation of transcription of the F-10 gene might involve steroid receptors.
Asunto(s)
Texto completo: 1 Índice: LILACS Asunto principal: Schistosoma mansoni / Proteínas del Huevo / Proteínas del Helminto / Proteínas de Unión al ADN Límite: Animals Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 1995 Tipo del documento: Article
Texto completo: 1 Índice: LILACS Asunto principal: Schistosoma mansoni / Proteínas del Huevo / Proteínas del Helminto / Proteínas de Unión al ADN Límite: Animals Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 1995 Tipo del documento: Article