Molecular aspects of Schistosoma mansoni female maturation
Mem. Inst. Oswaldo Cruz
;
90(2): 179-184, Mar.-Apr. 1995.
Artículo
en Inglés
| LILACS
| ID: lil-319903
ABSTRACT
Incubation of total protein extracts of Schistosoma mansoni with 3H 17-beta-estradiol and 20-hydroxyecdysone, revealed steroid binding proteins in both, male and female worms. The interaction of nuclear proteins with restriction fragments of the gender and stage-specific gene F-10 was investigated using the "Band-Shift" technique. Distinct male and female nuclear proteins bound to the fragments of this gene. Among the nuclear proteins, only those rich in cysteine residues bound to DNA. In vitro incubation of live worms with the estrogen antagonist Tamoxifen, altered the pattern of the DNA binding proteins, producing in females, a band profile similar to that obtained with male worm protein extracts. When Tamoxifen was injected into schistosome infected mice, the eggs produced by females presented an abnormal morphology, compatible with non-viable eggs. These results suggest that the regulation of transcription of the F-10 gene might involve steroid receptors.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Schistosoma mansoni
/
Proteínas del Huevo
/
Proteínas del Helminto
/
Proteínas de Unión al ADN
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
1995
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal do Rio de Janeiro/BR
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