UltramicroELISA indirecto para la detección de anticuerpos totales a Citomegalovirus en suero humano / Indirect ultramicroElisa for the detection of total antibodies against cytomegaloviruses in human blood
Rev. Inst. Med. Trop. Säo Paulo
;
34(1): 43-47, Jan.-Feb. 1992.
Artículo
en Español
| LILACS
| ID: lil-320631
RESUMO
We have standardized an indirect ultramicro ELISA assay for detecting antibodies to human Cytomegalovirus (CMV) using human serum samples (UMELISA CMV). The optimal concentration of coating antigen (30 ug/ml), serum dilution (140) and anti-human conjugate working dilution (11500), were determined by a check board titration method. The UMELISA CMV was compared with the latex agglutination test for antibodies to CMV (Dupont de Nemours) and with an indirect immunofluorescent method. The results have showed the high coincidence, sensitivity and especificity of the proposed assay regarding the two methods compared with, and supporting its use either for a blood donors screening or in the serological diagnosis of this infection by paired serum samples.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Citomegalovirus
/
Anticuerpos Antivirales
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Español
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Asunto de la revista:
Medicina Tropical
Año:
1992
Tipo del documento:
Artículo
País de afiliación:
Cuba
Institución/País de afiliación:
Banco de Sangue Provincial/CU
/
Centro de Inmunoensayo/CU
/
Instituto de Medicina Tropical Pedro Kouri Siboney/CU
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