Diagnosis of plague and identification of virulence markers in Yersinia pestis by multiplex-PCR
Rev. Inst. Med. Trop. Säo Paulo
;
41(6): 339-342, Nov.-Dec. 1999.
Artículo
en Inglés
| LILACS
| ID: lil-320657
RESUMO
We have developed a procedure for the rapid diagnosis of plague that also allows the identification of prominent virulence markers of Y. pestis strains. This procedure is based upon the use of a single polymerase chain reaction with multiple pairs of primers directed at genes present in the three virulence plasmids as well as in the chromosomal pathogenicity island of the bacterium. The technique allowed the discrimination of strains which lacked one or more of the known pathogenic loci, using as template total DNA obtained from bacterial cultures and from simulated blood cultures containing diluted concentration of bacteria. It also proved effective in confirming the disease in a blood culture from a plague suspected patient. As the results are obtained in a few hours this technique will be useful in the methodology of the Plague Control Program.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Peste
/
Yersinia pestis
/
Reacción en Cadena de la Polimerasa
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Asunto de la revista:
Medicina Tropical
Año:
1999
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Centro de Pesquisas Aggeu Magalhães/BR
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