Protective immunity of single and multi-antigen DNA vaccines against schistosomiasis
Mem. Inst. Oswaldo Cruz
;
97(suppl.1): 105-109, Oct. 2002. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-325012
RESUMO
We evaluated the usefulness of the combination of three plasmids encoding tegumental (pECL and pSM14) and muscular (pIRV5) antigens of the Schistosoma mansoni on improving the protective immunity over the use of a single antigen as DNA vaccines. Female BALB/c mice were inoculated twice with 25 æg DNA plasmid within two weeks interval. The challenge was performed with 80 cercarias of a regional isolate of S. mansoni (SLM) one week after the last immunization. Six weeks after challenge, all mice were perfused for worm load determination. The following groups were analyzed: saline; empty vector; monovalent formulations of pECL; pSM14 and pIRV5 and also double combinations of pECL/pIRV5 and pIRV5/pSM14 and a triple combination of pECL/pIRV5/pSM14. The protection was expressed as a percentage of worm loads in each group compared with the saline group. The results obtained were 41 percent (p < 0.05); 52 percent (p < 0.05); 51 percent (p < 0.05); 48 percent (p < 0.05); 55 percent (p < 0.05); 45 percent (p < 0.05); 65 percent (p < 0.05) for each group respectively
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Plásmidos
/
Schistosoma mansoni
/
Esquistosomiasis mansoni
/
Anticuerpos Antihelmínticos
/
Vacunas de ADN
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2002
Tipo del documento:
Artículo
/
Congreso y conferencia
País de afiliación:
Brasil
/
Estados Unidos
Institución/País de afiliación:
Fiocruz/BR
/
Johns Hopkins University/US
Similares
MEDLINE
...
LILACS
LIS