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Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection
Santos, Cecília Luiza Simöes dos; Bastos, Mariana Aparecida Antunes; Sallum, Maria Anice Mureb; Rocco, Iray Maria.
  • Santos, Cecília Luiza Simöes dos; Instituto Adolfo Lutz. Serviço de Virologia. São Paulo. BR
  • Bastos, Mariana Aparecida Antunes; Instituto Adolfo Lutz. Serviço de Virologia. São Paulo. BR
  • Sallum, Maria Anice Mureb; Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Epidemiologia. São Paulo. BR
  • Rocco, Iray Maria; Instituto Adolfo Lutz. Serviço de Virologia. São Paulo. BR
Rev. Inst. Med. Trop. Säo Paulo ; 45(1): 11-16, Jan.- Feb. 2003. ilus
Artículo en Inglés | LILACS | ID: lil-330506
ABSTRACT
In 2001, an autochthonous case of dual viremia, resulting from naturally acquired dengue virus DEN-1 and DEN-2 infections was detected during the dengue outbreak that occurred in Barretos, a city with about 105,000 inhabitants in the North region of São Paulo State. Serotype identification was based on virus isolation to C6/36 mosquito cells culture and immunofluorescence assays using type-specific monoclonal antibodies. The double infection was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Comparative analysis of the 240-nucleotide sequences of E/NS1 gene junction region between the genome of DEN-1 and DEN-2 isolates of the corresponding reference Nauru and PR 159S1 strains, respectively, showed some nucleotide differences, mainly silent mutations in the third codon position. Results of maximum likelihood phylogenetic analysis of E/NS1 gene sequences indicated that both genotypes of DEN-1 and DEN-2 viruses recovered from double infection in Barretos belonged to genotypes I and III, respectively
Asunto(s)
Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Dengue / Virus del Dengue Tipo de estudio: Estudio pronóstico Límite: Adolescente / Humanos / Masculino Idioma: Inglés Revista: Rev. Inst. Med. Trop. Säo Paulo Asunto de la revista: Medicina Tropical Año: 2003 Tipo del documento: Artículo / Documento de proyecto País de afiliación: Brasil Institución/País de afiliación: Instituto Adolfo Lutz/BR / Universidade de São Paulo/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Dengue / Virus del Dengue Tipo de estudio: Estudio pronóstico Límite: Adolescente / Humanos / Masculino Idioma: Inglés Revista: Rev. Inst. Med. Trop. Säo Paulo Asunto de la revista: Medicina Tropical Año: 2003 Tipo del documento: Artículo / Documento de proyecto País de afiliación: Brasil Institución/País de afiliación: Instituto Adolfo Lutz/BR / Universidade de São Paulo/BR