Expression and activity of matrix metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ
Braz. dent. j
;
13(2): 97-102, 2002.
Artículo
en Inglés
| LILACS
| ID: lil-332153
RESUMO
Tooth germ development is associated with morphological and biochemical changes of the dental papilla and enamel organ. Enzymes with gelatinolytic activities were studied by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography in tooth germ of newborn to 15-day-old rats. Three major bands with gelatinolytic activity were detected at all periods and characterized as the latent and active forms of MMP-2 using their molecular weight and activity dependent on Zn++ and Ca++ ions as criteria. Expression and activity of MMP-2 increased progressively from 0 to 15 days after birth. Mechanical separation of the tooth germ from 10-day-old rats showed that the gelatinolytic activity was localized mainly in the dental papilla and not the dental organ. These data indicate that the expression and activity of MMP-2 varies during the development and maturation of rat first molar tooth germ.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Germen Dentario
/
Metaloproteinasa 2 de la Matriz
/
Diente Molar
Límite:
Animales
Idioma:
Inglés
Revista:
Braz. dent. j
Asunto de la revista:
Odontología
Año:
2002
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
State University of Campinas-UNICAMP/BR
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