Expresión y caracterización de dos proteínas estructurales del virus de la diarrea viral bovina (VDVB) / Expression and characterization of 2 bovine viral diarrhea virus structural proteins (]BVDV)
Rev. argent. microbiol
;
33(1): 15-21, ene.-mar. 2001.
Artículo
en Español
| LILACS
| ID: lil-332506
RESUMO
The BVDV glycoproteins gp48 and gp53 were expressed in the baculovirus eukaryotic system. Both recombinant proteins were recognized in western blot analysis by monoclonal antibodies and polyclonal serum. Immunofluorescent test demonstrated that gp53 was localized on the cell surface whereas gp48 was in the cytoplasm. The expressed proteins were extracted by non-denaturing detergent treatment. Rabbit antiserum raised against gp53 recombinant protein efficiently neutralized the virus. These results demonstrate that the recombinant proteins have immunological properties similar to those of the native viral protein and that they can be useful as diagnostic reagents.
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Índice:
LILACS (Américas)
Asunto principal:
Proteínas del Envoltorio Viral
/
Virus de la Diarrea Viral Bovina
Límite:
Animales
Idioma:
Español
Revista:
Rev. argent. microbiol
Asunto de la revista:
Microbiologia
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
CICVyA/AR
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