Comparación de dos técnicas para el diagnóstico de laboratorio de infecciones por el virus influenza / Comparison of 2 techniques for the laboratory diagnosis of influenza virus infections
Rev. argent. microbiol
;
32(3): 144-148, jul.-sept. 2000.
Artículo
en Español
| LILACS
| ID: lil-332523
RESUMO
The clinical or epidemiological influenza diagnosis require fast, sensitive and accessible techniques for small laboratories. In order to investigate the sensitivity of the methods currently used in Argentina, the rapid diagnosis by indirect immunofluorescent assay (IF) was compared to the rapid viral culture in MDCK cells. The diagnosis of influenza virus infection was performed on 81 nasal and pharyngeal swabs collected from outpatients with upper respiratory infection, influenza-like syndrome. The samples were collected during 1998 winter season and both techniques were tested. The IF specificity and sensitivity obtained were 91.9 and 59.5, respectively. In the selection of the assay to be used for influenza diagnosis, the limitations of the simplest techniques such as IF should be considered. Furthermore, it is advisable to set up an optimized culture method in complex laboratories since culture is the only technique which allows the reference centers to perform the full characterization of the isolates.
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Índice:
LILACS (Américas)
Asunto principal:
Virus de la Influenza A
/
Faringe
/
Cultivo de Virus
/
Técnica del Anticuerpo Fluorescente Indirecta
/
Gripe Humana
/
Anticuerpos Antivirales
/
Cavidad Nasal
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Humanos
País/Región como asunto:
America del Sur
/
Argentina
Idioma:
Español
Revista:
Rev. argent. microbiol
Asunto de la revista:
Microbiologia
Año:
2000
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
Instituto Nacional de Enfermedades Infecciosas-ANLIS/AR
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