Metabolitos con actividad antiviral aislados de Curvularia pallescens / Antiviral activity metabolites isolated from Curvularia pallescens
Rev. argent. microbiol
;
31(4): 193-200, oct.-dec. 1999.
Artículo
en Portugués
| LILACS
| ID: lil-332548
RESUMEN
The strain BAFC 2336 of Curvularia pallescens is a hyphomycete isolated from internal fungi present in leaves and stems of Baccharis coridifolia. Three compounds designated B, D1 and D2 which inhibited the replication of vesicular stomatitis virus (VSV) and herpes simplex virus type 1 (HSV-1) in tissue cultures were isolated from fluid cultures of Curvularia pallescens. The purification procedure of the compounds consisted first in an organic solvent extraction followed by chromatography through a silica gel column. Fractions eluted from the column with antiviral activity were pooled and then subjected to thin layer chromatography (TLC) in silica gel plates. Three isolated bands were recognized with Rf of 0.63, 0.36 y 0.33 for B, D1 and D2, respectively. The chromatographic characteristics of the isolated metabolites were determined by TLC and HPLC and their chemical structure by means of spectroscopic methods. Analysis of the data obtained indicated that compound D2 (MW 280 Da) is identical with Brefeldin A a macrolide with antiviral activity isolated from other fungi but not reported to be present in Curvularia pallescens. Compound D1 is similar in structure to compound D2; however, the low amount obtained after purification unabled us to obtain complete structural characterization. Compound B (MW 332 Da) has an aromatic ring and a chemical structure related to curvularins, a generic name for certain metabolites from Curvularia. This compound appears to be a novel compound with antiviral potency similar to Brefeldin A, but less toxic.
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Índice:
LILACS (Américas)
Asunto principal:
Antivirales
/
Hongos Mitospóricos
Idioma:
Portugués
Revista:
Rev. argent. microbiol
Asunto de la revista:
Microbiologia
Año:
1999
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
Universidad de Buenos Aires/AR
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