Comportamiento biológico de 3 cepas del virus dengue-2 en 2 líneas celulares de mosquitos / Biological behavior of 3 strains of the dengue-2 virus in 2 cell lines of mosquitoes
Rev. cuba. med. trop
;
52(3): 215-219, Sept.-Dec. 2000.
Artículo
en Español
| LILACS
| ID: lil-333468
ABSTRACT
Strains A-15 (isolated in Cuba, 1981), Jamaica (isolated in Jamaica, 1981) and Nueva Guinea "C" (standard) from dengue-2 virus were compared according to the time of appearance of the cytopathic effect (CPE), to the time of appearance of specific fluorescence and to the kynetics of viral multiplication on being innoculated in the cell lines AP-61 (Aedes pseudoscutellaris) and C6/36 HT (Aedes albopictus). The results showed that the CPE of highest intensity and earliest appearance was for A-15, followed by Jamaica and Nueva Guinea "C" (NGC). AP-61 seems to favor the CPE of Jamaica with respect to that of the same strain in C6/36 HT. The fluorescence was earlier for Jamaica and A-15 and more intensive for the latter, whereas NGC manifested late. This behaviour was similar in the 2 cellular systems. The greatest titres during the kinetics of viral multiplication were obtained from A-15 in both lines, although in AP-61 they tend to be equal from the 4th day on. The strain A-15 showed a particular behaviour of these biological properties on comparing them with the other strains under study, which may be related to changes found in its neucleotide sequence.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Aedes
/
Virus del Dengue
Límite:
Animales
Idioma:
Español
Revista:
Rev. cuba. med. trop
Asunto de la revista:
Medicina Tropical
Año:
2000
Tipo del documento:
Artículo
País de afiliación:
Cuba
/
Venezuela
Institución/País de afiliación:
Instituto Medicina Tropical Pedro Kourí/CU
/
Laboratorio de referencia y diagnóstico de dengue y otras enfermedades virales/VE
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