Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
Mem. Inst. Oswaldo Cruz
;
98(2): 269-271, Mar. 15, 2003. ilus, graf
Artículo
en Inglés
| LILACS
| ID: lil-334266
ABSTRACT
We describe a method for culturing over 90 percent pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Trypanosoma
/
Técnicas de Cultivo de Célula
/
Macrófagos
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2003
Tipo del documento:
Artículo
País de afiliación:
Colombia
Institución/País de afiliación:
Universidad de Antioquia/CO
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