Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies
Mem. Inst. Oswaldo Cruz
;
98(2): 287-290, Mar. 15, 2003. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-334270
ABSTRACT
Phlebotominae sand fly specimens were prepared for histological and physiological studies. Different fixatives were tested on sectioned and whole bodied adult females in order to obtain good fixation and provide satisfactory penetration of the embedding media. All fixed specimens were infiltrated (up to seven days under 5ºC) and embedded in hydroxyethyl metacrylate. Two-three æm sections were stained, mounted in Canada balsam and observed by light microscopy. Best results were achieved when whole bodied insects were double fixed in Bouin's and Carnoy's fluids (4 h/2 h) and stained in Hematoxilin/Eosin or fixed in calcium formaldehyde and stained in mercury bromophenol blue
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Psychodidae
/
Fijación del Tejido
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2003
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Fiocruz/BR
/
Universidade Federal de Viçosa/BR
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