Identification of new isolates of Bacillus thuringiensis using rep-PCR products and d-endotoxin electron microscopy
Genet. mol. biol
;
25(2): 225-229, Jun. 2002. ilus
Artículo
en Inglés
| LILACS
| ID: lil-335793
ABSTRACT
PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Bacillus thuringiensis
/
Proteínas Bacterianas
/
Reacción en Cadena de la Polimerasa
/
Endotoxinas
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Idioma:
Inglés
Revista:
Genet. mol. biol
Asunto de la revista:
Genética
Año:
2002
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
UNESP/BR
/
USP/BR
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