Unilateral enucleation induces an increase of 160 kd neurofilament in lateral geniculate nuclei synapses
Biocell
;
20(1): 55-59, Apr. 1996.
Artículo
en Inglés
| LILACS
| ID: lil-336005
RESUMO
Ultrastructural synaptic changes of retinal origin in the pars dorsalis lateral geniculate nuclei (dLGN) after enucleation have been studied in this laboratory, showing a filamentous hypertrophy with maximal expression at 4-6 days post-lesion in monkeys (Pecci Saavedra et al., 1970, 1971). The aim of this work was to elucidate the nature of the newly formed filament in dLGN in post-enucleated rats. Male Wistar rats were fixed with 4 paraformaldehyde plus 0.25 glutaraldehyde in 0.1M phosphate buffer, through the abdominal aorta after 3, 5, and 7 days postenucleation. Sections obtained were incubated with antibodies to the phosphorylated portion of the 160 Kd neurofilaments (13000) and anti-GFAP (125000). There was an increase in 160 Kd neurofilament staining in axons and degenerating nerve endings in dLGN, as well as a typical astroglial immunostained reaction. Our results show that the newly formed neurofilaments after deafferentation are of the 160 Kd type, commonly present in normal axons.
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Índice:
LILACS (Américas)
Asunto principal:
Sinapsis
/
Enucleación del Ojo
/
Proteínas de Neurofilamentos
/
Cuerpos Geniculados
Límite:
Animales
Idioma:
Inglés
Revista:
Biocell
Asunto de la revista:
Clulas
Año:
1996
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
Universidad de Buenos Aires/AR
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