Serotonin reverses the inhibitory effect of a brain soluble fraction on synaptosomal membrane Na+, K(+)-ATPase activity
Biocell
;
19(2): 153-157, Aug. 1995.
Artículo
en Inglés
| LILACS
| ID: lil-336012
ABSTRACT
Normal operation of the Na+ pump (Na+, K(+)-ATPase) is essential for the maintenance of neurotransmission. Filtration through Sephadex G-50 of a brain soluble fraction allowed the separation of peaks I and II fractions, respectively stimulating and inhibiting synaptosomal membrane Na+, K(+)-ATPase activity. Peaks I and II were isolated from rat cerebral cortex and their effect together with serotonin (5-HT) was studied on ATPase activity by estimating K(+)-p-nitrophenylphosphatase activity in brain cortex synaptosomal membranes. It was observed that 10(-5) or 10(-4) M 5-HT failed to modify either control membrane enzyme activity or peak I activatory effect; on the other hand, such 5-HT concentrations significantly suppressed peak II inhibitory effect. This ability of 5-HT to reverse the inhibitory effect of endogenous factors on Na+, K(+)-ATPase activity could well be a new 5-HT modulatory action within the brain.
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Índice:
LILACS (Américas)
Asunto principal:
Sinaptosomas
/
Técnicas In Vitro
/
Serotonina
/
ATPasa Intercambiadora de Sodio-Potasio
Límite:
Animales
Idioma:
Inglés
Revista:
Biocell
Asunto de la revista:
Clulas
Año:
1995
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
Universidad de Buenos Aires/AR
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