Preservation of goat preantral follicles in saline or coconut water solution
Braz. j. vet. res. anim. sci
;
39(6): 324-330, 2002. ilus, graf
Artículo
en Inglés
| LILACS
| ID: lil-337385
RESUMO
The present study investigated the efficiency of saline solution and coconut water solution in the preservation of goat preantral follicles enclosed in ovarian tissue, at different temperatures and for different incubation periods. At the slaughterhouse, the ovarian pair was divided into 19 fragments; one ovarian fragment was immediately fixed for histology (control-time zero). The other 18 ovarian fragments were preserved in both solutions at 4ºC, 20ºC or 39ºC for 4 h, 12 h or 24 h. The histological analysis showed that the storage of ovarian fragments in both solutions at 4ºC for up to 24 h kept the percentage of normal preantral follicles similar to the control values. In contrast, preservation at 20°C or 39ºC, in either solution, reduced significantly the percentage of normal preantral follicles compared to the control values, except in saline solution at 20ºC for 4 h or in coconut water solution at 20ºC for 4 h and 12 h. In conclusion, this study shows that both solutions can be used with the same efficiency to preserve goat preantral follicles at 4°C, irrespective of the incubation time. However, to preserve goat preantral follicles at higher temperatures, coconut water solution is recommended
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Índice:
LILACS (Américas)
Asunto principal:
Cabras
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Folículo Ovárico
Idioma:
Inglés
Revista:
Braz. j. vet. res. anim. sci
Asunto de la revista:
Medicina Veterinaria
Año:
2002
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Estadual do Ceará/BR
/
Universidade Federal do Pará/BR
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