Nuevos mecanismos involucrados en la patogenesis de adenomas hipofisarios / New mechanisms involved in the pathogenesis of pituiatary adenomas
Medicina (B.Aires)
;
63(2): 147-150, 2003. tab, graf
Artículo
en Español
| LILACS
| ID: lil-338581
ABSTRACT
We studied Smad-4dn tumors generated from lactosomatotrophic GH3 cells stably transfected with a dominant negative form of Smad-4 (a bone morphogenetic protein-4, BMP-4, signal co-transducer) which had reduced tumorigenicity in nude mice, but had showed a late increase in tumor size. We found that they had lost in vivo the expression of Smad-4dn and had recovered c-Myc expression. In accordance, BMP-4 is overexpressed and stimulates the expression of c-Myc in human prolactinomas, but not in other human pituitary adenomas or normal pituitary. In addition ICI 182,780 inhibited BMP-4 stimulated c-Myc expression and BMP-4 and 17 beta-estradiol in combination had an additive effect on GH3 cell proliferation. Their action was inhibited by blocking BMP-4 with ICI 182,780 or 17 beta-estradiol with Smad-4dn. Furthermore, co-immunoprecipitation studies demonstrate that Smad-4 physically interacts with the ER alpha/ER beta. We show for the first time the role of BMP-4 in prolactinoma pathogenesis, involving a functional cross-talk BMP-4/E2
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Neoplasias Hipofisarias
/
Prolactinoma
/
Proteínas Morfogenéticas Óseas
Tipo de estudio:
Estudio de etiología
Límite:
Animales
/
Humanos
Idioma:
Español
Revista:
Medicina (B.Aires)
Asunto de la revista:
Medicina
Año:
2003
Tipo del documento:
Artículo
/
Congreso y conferencia
País de afiliación:
Argentina
Institución/País de afiliación:
Consejo Nacional de Investigaciones Científicas y Técnicas/AR
/
Hospital Santa Lucía/AR
/
Universidad de Buenos Aires/AR
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