A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
Mem. Inst. Oswaldo Cruz
;
98(3): 387-390, Apr. 2003. ilus
Artículo
en Inglés
| LILACS
| ID: lil-340120
ABSTRACT
Polytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20ºC provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic acid. A simple technique should facilitate molecular cytogenetics used in the location of resistance and vector competence genes
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Mapeo Cromosómico
/
Aedes
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2003
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Estadual de Campinas/BR
Similares
MEDLINE
...
LILACS
LIS