Isolation of a frog lectin using polylysine to reduce non-specific interactions with heparin binding ligand
Biocell
;
22(3): 163-166, Dec. 1998.
Artículo
en Inglés
| LILACS
| ID: lil-340392
ABSTRACT
Polylysine is a polycation used in histology to glue tissue sections to glass slides. It was successfully used to eliminate positively charged proteins from heparin-agarose beds applied for affinity chromatography facilitating the preparative steps in the isolation and purification of heparin binding lectins from the ovary of the frog Leptodactylus occellatus. The addition pretreatment of agarose-heparin beds with polylysine increased more than 3-fold the hemagglutinating capacity of eluted lectin
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Índice:
LILACS (Américas)
Asunto principal:
Anuros
/
Polilisina
/
Sitios de Unión
/
Heparina
/
Cromatografía de Afinidad
/
Lectinas
Límite:
Animales
Idioma:
Inglés
Revista:
Biocell
Asunto de la revista:
Clulas
Año:
1998
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
Universidad Nacional de Córdoba/AR
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