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Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis
Silva, Luciana Pereira; Barcelos, Ivanildes Solange da Costa; Passos-Lima, Andréia Barcelos; Espindola, Foued Salmen; Campos, Dulcinéa Maria Barbosa; Costa-Cruz, Julia Maria.
Afiliación
  • Silva, Luciana Pereira; Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Departamento de Imunologia, Microbiologia e Parasitologia. Uberlândia. BR
  • Barcelos, Ivanildes Solange da Costa; Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Departamento de Imunologia, Microbiologia e Parasitologia. Uberlândia. BR
  • Passos-Lima, Andréia Barcelos; Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia. BR
  • Espindola, Foued Salmen; Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia. BR
  • Campos, Dulcinéa Maria Barbosa; Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Departamento de Parasitologia. Goiânia. BR
  • Costa-Cruz, Julia Maria; Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Departamento de Imunologia, Microbiologia e Parasitologia. Uberlândia. BR
Mem. Inst. Oswaldo Cruz ; 98(5): 687-691, July 2003. ilus, graf
Article en En | LILACS | ID: lil-344290
Biblioteca responsable: BR1.1
RESUMO
The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5 percent of the cases of strongyloidiasis. The negative concordance in the three tests was 94 percent and 97.5 percent, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology
Asunto(s)
Texto completo: 1 Índice: LILACS Asunto principal: Estrongiloidiasis / Anticuerpos Antihelmínticos / Strongyloides ratti / Antígenos Helmínticos Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 2003 Tipo del documento: Article
Texto completo: 1 Índice: LILACS Asunto principal: Estrongiloidiasis / Anticuerpos Antihelmínticos / Strongyloides ratti / Antígenos Helmínticos Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 2003 Tipo del documento: Article