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Factors involved with cadmium absorption by a wild-type strain of Saccharomyces cerevisiae
Adamis, Paula D. B; Panek, Anita D; Leite, Selma G. F; Eleutherio, Elis C. A.
  • Adamis, Paula D. B; Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro. BR
  • Panek, Anita D; Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro. BR
  • Leite, Selma G. F; Escola de Química. Rio de Janeiro. BR
  • Eleutherio, Elis C. A; Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro. BR
Braz. j. microbiol ; 34(1): 55-60, Jan.-Apr. 2003. graf
Artículo en Inglés | LILACS | ID: lil-344566
ABSTRACT
At the concentration used in this work (10 ppm), cadmium was efficiently removed from the environment by stationary yeast cells. While exponential phase cells showed low capacity of cadmium absorption, stationary cells removed 97 percent of the original metal in 24 hours. Total cadmium absorption shown by dry cells was lower than that of fresh ones, although both cells removed 50 percent of metal during the first hour of treatment. We also verified that only viable cells were capable of absorbing cadmium. Independently of the growth phase, cells showed high tolerance to 10 ppm CdSO4 and about 80 percent of cells remained viable after 24 hours exposure to cadmium. However, when stationary phase cells were previously dehydrated and then exposed to cadmium, they exhibited poor survival. By using an oxidation-dependent fluorescent probe, we observed that, once absorbed by cells, cadmium increases the intracellular level of oxidation, which may be responsible for its toxic effect. Crude extracts from stationary phase cells exposed to cadmium showed a 10-fold increase in fluorescence, while extracts from cells of exponential phase did not increase in fluorescence. Dry cells treated with the metal showed a high increase in fluorescence, mainly caused by dehydration.
Asunto(s)
Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Saccharomyces cerevisiae / Técnicas In Vitro / Cadmio / Oxidación Biológica Idioma: Inglés Revista: Braz. j. microbiol Asunto de la revista: Microbiologia Año: 2003 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Escola de Química/BR / Universidade Federal do Rio de Janeiro/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Saccharomyces cerevisiae / Técnicas In Vitro / Cadmio / Oxidación Biológica Idioma: Inglés Revista: Braz. j. microbiol Asunto de la revista: Microbiologia Año: 2003 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Escola de Química/BR / Universidade Federal do Rio de Janeiro/BR