PCR detection of four periodontopathogens from subgingival clinical samples
Braz. j. microbiol
;
34(1): 81-84, Jan.-Apr. 2003. tab
Artículo
en Inglés
| LILACS
| ID: lil-344571
RESUMO
In this study, A. actinomycetemcomitans, B. forsythus, P. gingivalis and F. nucleatum were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. Subgingival clinical samples were collected with sterilized paper points and transported in VMGA III. From all the diluted clinical samples (1:10), DNA was obtained by boiling, and after centrifugation the supernatant was used as template. Specific primers for each bacterial species were used in PCR. PCR amplification was sensitive to identify these organisms. PCR products from each species showed a single band and can be used to identify periodontal organisms from clinical specimens. PCR detection odds ratio values for A. actinomycetemcomitans and B. forsythus were significantly associated with disease showing a higher OR values for B. forsythus (2.97, 95 percent CI 1.88 - 4.70). These results suggest a strong association among the studied species and the periodontal lesion.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Enfermedades Periodontales
/
Técnicas In Vitro
/
Reacción en Cadena de la Polimerasa
/
Patógenos Transmitidos por la Sangre
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2003
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade de São Paulo/BR
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