Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
Genet. mol. biol
;
26(3): 329-336, 2003. ilus, tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-346324
ABSTRACT
The RAPD technique associated with restriction digestion of genomic DNA was used to assess the genetic variability within and among nine populations of Coffea arabica, including six progenies belonging to the Sarchimor germplasm, the progeny PR 77054-40-10 (Catuaí Vermelho IAC 81 x Icatu), and two commercial cultivars (IAPAR 59 and Catuaí Vermelho IAC-81). These populations were evaluated using analysis of molecular variance (AMOVA), genetic similarity among progenies, and percentage of polymorphic loci. A total of 99 RAPD markers were evaluated of which 67 (67.67 percent) were polymorphic. AMOVA showed that 38.5 percent and 61.5 percent of the genetic variation was distributed among and within populations, respectively. The fixation index (F ST) of the genotypes was 0.385. The mean genetic variability estimated within populations ranged from 15.58 (IAPAR 59) to 8.27 (Catuaí Vermelho IAC 81). A distinct level of genetic variability was revealed for each of the coffee progenies and varieties studied. The methodology used in this investigation was useful to determine the genetic variability within and among C. arabica L. populations providing significant information for coffee breeding
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Polimorfismo Genético
/
Variación Genética
/
Café
/
Técnica del ADN Polimorfo Amplificado Aleatorio
Idioma:
Inglés
Revista:
Genet. mol. biol
Asunto de la revista:
Genética
Año:
2003
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Instituto Agronômico do Paraná/BR
/
Universidade Estadual de Londrina/BR
/
Universidade Federal de Goias/BR
Similares
MEDLINE
...
LILACS
LIS