An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
Mem. Inst. Oswaldo Cruz
;
98(7): 915-917, Oct. 2003. ilus
Artículo
en Inglés
| LILACS
| ID: lil-352394
RESUMO
Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Toxoplasma
/
Encéfalo
/
Toxoplasmosis Animal
/
Quistes
/
Amilopectina
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2003
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Fiocruz/BR
/
Universidade do Estado do Rio de Janeiro/BR
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