Plasminogen interaction with Trypanosoma cruzi
Mem. Inst. Oswaldo Cruz
;
99(1): 63-67, Feb. 2004. ilus, graf
Artículo
en Inglés
| LILACS
| ID: lil-356445
RESUMO
The ability of Trypanosoma cruzi to interact with plasminogen, the zimogenic form of the blood serin protease plasmin, was examined. Immunohistochemistry studies revealed that both forms, epimastigotes and metacyclic trypomastigotes, were able to fix plasminogen in a lysine dependant manner. This interaction was corroborated by plasminogen activation studies. Both forms of the parasite enhanced the plasminogen activation by tissue-type plasminogen activator.The maximal enhancements obtained were 15-fold and 3.4-fold with epimastigotes and metacyclic trypomastigotes, respectively, as compared to plasminogen activation in absence of cells. Ligand-blotting analysis of proteins extracted with Triton X-114 from a microsomal fraction of epimastigotes revealed at least five soluble proteins and one hydrophobic protein able to bind plasminogen.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Plasminógeno
/
Trypanosoma cruzi
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2004
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Venezuela
Institución/País de afiliación:
Universidad de Los Andes/VE
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