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Modulation of hormone secretion by functional electrical stimulation of the intact and incompletely dysfunctional dog pancreas
Rozman, J; Bunc, M; Zorko, B.
  • Rozman, J; University of Ljubljana. Center for Implantable Technology and Sensors. ITIS d.o.o. Ljubljana. Ljubljana. SI
  • Bunc, M; University of Ljubljana. Institute of Pathophysiology. School of Medicine. Ljubljana. SI
  • Zorko, B; University of Ljubljana. Veterinary Faculty. Ljubljana. SI
Braz. j. med. biol. res ; 37(3): 363-370, Mar. 2004. ilus, tab
Artículo en Inglés | LILACS | ID: lil-356616
ABSTRACT
The purpose of the present study was to modulate the secretion of insulin and glucagon in Beagle dogs by stimulation of nerves innervating the intact and partly dysfunctional pancreas. Three 33-electrode spiral cuffs were implanted on the vagus, splanchnic and pancreatic nerves in each of two animals. Partial dysfunction of the pancreas was induced with alloxan. The nerves were stimulated using rectangular, charge-balanced, biphasic, and constant current pulses (200 µs, 1 mA, 20 Hz, with a 100-µs delay between biphasic phases). Blood samples from the femoral artery were drawn before the experiment, at the beginning of stimulation, after 5 min of stimulation, and 5 min after the end of stimulation. Radioimmunoassay data showed that in the intact pancreas stimulation of the vagal nerve increased insulin (+99.2 µU/ml) and glucagon (+18.7 pg/ml) secretion and decreased C-peptide secretion (-0.15 ng/ml). Splanchnic nerve stimulation increased insulin (+1.7 µU/ml), C-peptide (+0.01 ng/ml), and glucagon (+50 pg/ml) secretion, whereas pancreatic nerve stimulation did not cause a marked change in any of the three hormones. In the partly dysfunctional pancreas, vagus nerve stimulation increased insulin (+15.5 µU/ml), glucagon (+11 pg/ml), and C-peptide (+0.03 ng/ml) secretion. Splanchnic nerve stimulation reduced insulin secretion (-2.5 µU/ml) and increased glucagon (+58.7 pg/ml) and C-peptide (+0.39 ng/ml) secretion, and pancreatic nerve stimulation increased insulin (+0.2 µU/ml), glucagon (+5.2 pg/ml), and C-peptide (+0.08 ng/ml) secretion. It was concluded that vagal nerve stimulation can significantly increase insulin secretion for a prolonged period of time in intact and in partly dysfunctional pancreas.
Asunto(s)
Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Páncreas / Glucagón / Estimulación Eléctrica / Insulina Límite: Animales Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 2004 Tipo del documento: Artículo / Documento de proyecto País de afiliación: Eslovenia Institución/País de afiliación: University of Ljubljana/SI

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Páncreas / Glucagón / Estimulación Eléctrica / Insulina Límite: Animales Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 2004 Tipo del documento: Artículo / Documento de proyecto País de afiliación: Eslovenia Institución/País de afiliación: University of Ljubljana/SI