Detección molecular de calcivirus entericos de bovino en Venezuela / Molecular identification of bovine enteric calciviruses in Venezuela

ABSTRACT

Caliciviruses are a well-established cause of respiratory, vesicular and hemorrhagic diseases in animals. In addition, these viruses are an important cause of enteric diseases in humans. Recently, molecular analysis of several bovine enteric calicivirus isolates indicated that they are genetically close to human enteric calicivirus. To investigate if bovine enteric caliciviruses circulate in Venezuela, 129 stool samples collected between 1994 and 2000 were assayed by reverse transcription-polymerase chain reaction amplification. The presence of calicivirus was confirmed in one of the samples analyzed, collected in the Lara State from a healthy calf, 2 months old. Phylogenetic studies based on partial RNA polymerase sequences indicated that the Venezuelan isolate (Bo/NV/Lara/2000/VE) is most closely related to the genogroup III, genus Noroviruses.