Immunoblotting for the serodiagnosis of Helicobacter pylori áinfection in Brazilian patients with and without gastric carcinoma
Mem. Inst. Oswaldo Cruz
;
99(2): 189-193, Mar. 2004. tab
Artículo
en Inglés
| LILACS
| ID: lil-360974
ABSTRACT
We evaluated the performance of a commercial immunoblotting in the serodiagnosis of Helicobacter pylori infection in Brazilian patients. The presence of anti-H. pylori antibodies was also investigated in a group of 20 duodenal ulcer patients after successful treatment. One hundred and ninety one patients were studied. Among the 164 infected patients, 46 had gastric carcinoma. The duodenal ulcer patients were treated with antimicrobial drugs and the eradication of the microorganism was confirmed in all of them one month after the end of the treatment by the 13C-urea breath test. Sera were assayed for H. pylori antibodies using the Helicoblot 2.0 (Genelabs Diagnostics, Singapore). The sensitivity, specificity, positive, and negative predictive values of the test were 93.9 percent, 92.6 percent, 98.7 percent, and 71.4 percent, respectively. The sensitivity of the test was similar in patients with (93.5 percent) and without (95.7 percent) gastric carcinoma. Twenty-four months after the end of the treatment, the band of 116 kDa was still detected in one of the patients. In conclusion, the Helicoblot 2.0 is an accurate test to diagnose H. pylori infection and although it can not be employed to monitor the bacterium eradication, it may be useful for diagnosing past infection, especially in gastric carcinoma patients.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Immunoblotting
/
Helicobacter pylori
/
Infecciones por Helicobacter
/
Úlcera Duodenal
/
Anticuerpos Antibacterianos
Tipo de estudio:
Estudio diagnóstico
Límite:
Adolescente
/
Adulto
/
Anciano
/
Aged80
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2004
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de Minas Gerais/BR
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