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Production of recombinant proteins in Escherichia coli
Schumann, Wolfgang; Ferreira, Luis Carlos S.
  • Schumann, Wolfgang; University of Bayreuth. Institute of Genetics. Bayreuth. DE
  • Ferreira, Luis Carlos S; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia. São Paulo. BR
Genet. mol. biol ; 27(3): 442-453, Sept. 2004. tab
Artículo en Inglés | LILACS | ID: lil-366190
RESUMO
Attempts to obtain a recombinant protein using prokaryotic expression systems can go from a rewarding and rather fast procedure to a frustrating time-consuming experience. In most cases production of heterologous proteins in Escherichia coli K12 strains has remained an empirical exercise in which different systems are tested without a careful insight into the various factors affecting adequate expression of the encoded protein. The present review will deal with E. coli as protein factory and will cover some of the aspects related to transcriptional and translational expression signals, factors affecting protein stability and solubility and targeting of proteins to different cell compartments. Based on the knowledge accumulated over the last decade, we believe that the rate of success for those dedicated to expression of recombinant proteins based on the use E. coli strains can still be significantly improved.
Asunto(s)
Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Proteínas Recombinantes / Secuencia de Bases / Escherichia coli Idioma: Inglés Revista: Genet. mol. biol Asunto de la revista: Genética Año: 2004 Tipo del documento: Artículo País de afiliación: Brasil / Alemania Institución/País de afiliación: Universidade de São Paulo/BR / University of Bayreuth/DE

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Proteínas Recombinantes / Secuencia de Bases / Escherichia coli Idioma: Inglés Revista: Genet. mol. biol Asunto de la revista: Genética Año: 2004 Tipo del documento: Artículo País de afiliación: Brasil / Alemania Institución/País de afiliación: Universidade de São Paulo/BR / University of Bayreuth/DE