Production of recombinant proteins in Escherichia coli
Genet. mol. biol
;
27(3): 442-453, Sept. 2004. tab
Artículo
en Inglés
| LILACS
| ID: lil-366190
RESUMO
Attempts to obtain a recombinant protein using prokaryotic expression systems can go from a rewarding and rather fast procedure to a frustrating time-consuming experience. In most cases production of heterologous proteins in Escherichia coli K12 strains has remained an empirical exercise in which different systems are tested without a careful insight into the various factors affecting adequate expression of the encoded protein. The present review will deal with E. coli as protein factory and will cover some of the aspects related to transcriptional and translational expression signals, factors affecting protein stability and solubility and targeting of proteins to different cell compartments. Based on the knowledge accumulated over the last decade, we believe that the rate of success for those dedicated to expression of recombinant proteins based on the use E. coli strains can still be significantly improved.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Proteínas Recombinantes
/
Secuencia de Bases
/
Escherichia coli
Idioma:
Inglés
Revista:
Genet. mol. biol
Asunto de la revista:
Genética
Año:
2004
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
Alemania
Institución/País de afiliación:
Universidade de São Paulo/BR
/
University of Bayreuth/DE
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