Development of an experimental model of neutrophilic pulmonary response induction in mice
J. pneumol
; 29(4): 213-216, jul.-ago. 2003. graf
Article
en En
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| ID: lil-366307
Biblioteca responsable:
BR674.1
RESUMO
BACKGROUND:
Several lung diseases are characterized by a predominantly neutrophilic inflammation. A better understanding of the mechanisms of action of some drugs on the airway inflammation of such diseases may bring advances to the treatment.OBJECTIVE:
To develop a method to induce pulmonary neutrophilic response in mice, without active infection.METHODS:
Eight adult Swiss mice were used. The study group (n = 4) received an intranasal challenge with 1 x 10(12) CFU/ml of Pseudomonas aeruginosa (Psa), frozen to death. The control group (n = 4) received an intranasal challenge with saline solution. Two days after the intranasal challenge, a bron¡choalveolar lavage (BAL) was performed with total cell and differential cellularity counts.RESULTS:
The total cell count was significantly higher in the group with Psa, as compared to the control group (median of 1.17 x 10(6) and 0.08 x 10(6), respectively, p = 0.029). In addition to this, an absolute predominance of neutrophils was found in the differential cellularity of the mice that had received the Psa challenge.CONCLUSIONS:
The model of inducing a neutrophilic pulmonary disease using frost-dead bacteria was successfully developed. This neutrophilic inflammatory response induction model in Swiss mice lungs may be an important tool for testing the anti-inflammatory effect of some antimicrobial drugs on the inflammation of the lower airways.
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Índice:
LILACS
Asunto principal:
Pseudomonas aeruginosa
/
Infecciones por Pseudomonas
/
Activación Neutrófila
/
Enfermedades Pulmonares
Límite:
Animals
Idioma:
En
Revista:
J. pneumol
Asunto de la revista:
PNEUMOLOGIA
Año:
2003
Tipo del documento:
Article