Leishmania infantum heat shock protein 83 for the serodiagnosis of tegumentary leishmaniasis
Braz. j. med. biol. res
;
37(11): 1591-1593, Nov. 2004. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-385863
RESUMO
The serologic assay is an important tool in the diagnosis of leishmaniasis. One of the most commonly used tests is enzyme-linked immunosorbent assay (ELISA). Since total Leishmania promastigotes are used as antigen in the routine assay, false-positive reactions are frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, an antigen that determines less cross-reactivity has been pursued for the serodiagnosis of leishmaniasis. In the present study we analyzed the use of recombinant Leishmania infantum heat shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous (N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed the presence of anti-L. infantum Hsp 83 antibodies in all samples as well as anti-Leishmania total antigen antibodies. When cross-reactivity was tested, chronic Chagas' disease patients (N = 10) did not show any reactivity. Therefore, we consider this L. infantum Hsp 83 to be a good antigen for routine use for serodiagnosis of tegumentary leishmaniasis.
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Índice:
LILACS (Américas)
Asunto principal:
Anticuerpos Antiprotozoarios
/
Proteínas Protozoarias
/
Leishmaniasis Cutánea
/
Leishmania infantum
/
Proteínas de Choque Térmico
/
Antígenos de Protozoos
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
2004
Tipo del documento:
Artículo
/
Congreso y conferencia
/
Documento de proyecto
País de afiliación:
Argentina
/
Brasil
Institución/País de afiliación:
ANLIS Dr. Carlos G. Malbran/AR
/
Universidade de São Paulo/BR
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