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Protein engineering of delta-endotoxins of Bacillus thuringiensis
Saraswathy, Nachimuthu; Kumar, Polumetla Ananda.
  • Saraswathy, Nachimuthu; Indian Agricultural Research Institute. National Research Centre for Plant Biotechnology. New Delhi. IN
  • Kumar, Polumetla Ananda; Indian Agricultural Research Institute. National Research Centre for Plant Biotechnology. New Delhi. IN
Electron. j. biotechnol ; 7(2): 178-188, Aug. 2004. ilus, tab
Artículo en Inglés | LILACS | ID: lil-387556
ABSTRACT
Bacillus thuringiensis (Bt) is a valuable environment-friendly biopesticide, which occupies 90 percent of the world biopesticide market. Its insecticidal properties are attributed to the presence of delta-endotoxins which are synthesized during the sporulation phase of the bacterium. delta-endotoxin or crystal toxin is a multi-domain protein molecule comprising of three distinct domains. Domain I is made of seven alpha-helices, domain II comprises three antiparallel beta sheets, which are folded into loops and domain III is made of a beta sandwich of two antiparallel beta strands. Molecular studies on the structure and functional properties of different delta-endotoxins revealed that the domain I by virtue of its membrane spanning hydrophobic and amphipathic alpha-helices is capable of forming pores in the cell membranes of the larval midgut. Domain II being hyper variable in nature determines the insecticidal specificity of a toxin and domain III is involved in varied functions like structural stability, ion channel gating, binding to Brush Border Membrane Vesicles and insecticidal specificity. Recent studies on toxin aggregation and interaction revealed that the three domains interact closely to bring about the insecticidal activity of Bt. In this review we describe the protein engineering studies conducted on different delta-endotoxins which led to an understanding of their molecular mode of action and construction of novel toxins with enhanced insecticidal activity and specificity.
Asunto(s)
Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Bacillus thuringiensis / Proteínas Bacterianas / Toxinas Bacterianas / Ingeniería de Proteínas Idioma: Inglés Revista: Electron. j. biotechnol Asunto de la revista: Biotecnologia Año: 2004 Tipo del documento: Artículo País de afiliación: India Institución/País de afiliación: Indian Agricultural Research Institute/IN

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Bacillus thuringiensis / Proteínas Bacterianas / Toxinas Bacterianas / Ingeniería de Proteínas Idioma: Inglés Revista: Electron. j. biotechnol Asunto de la revista: Biotecnologia Año: 2004 Tipo del documento: Artículo País de afiliación: India Institución/País de afiliación: Indian Agricultural Research Institute/IN