In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque
Mem. Inst. Oswaldo Cruz
;
99(6): 629-631, Oct. 2004. ilus
Artículo
en Inglés
| LILACS
| ID: lil-387914
ABSTRACT
The liver tissue of a rhesus macaque inoculated with hepatitis C virus (HCV) has been analyzed for the presence of HCV RNA using the technique of in situ hybridization, both at light and electron microscopy levels. The animal was inoculated by the intrasplenic route using a HCV infected autogenic hepatocyte transplant. The serum sample used to infect the hepatocyte cells was characterized by polymerase chain reaction technique and shown to be positive for HCV RNA, genotype 3 with 10(7) RNA copies/ml. In situ hybridization was performed using a complementary negative strand probe made with the specific primer. We were able to detect and localize viral RNA in altered membranes of the rough endoplasmic reticulum of infected liver cells, showing evidence of virus replication in vivo.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
ARN Viral
/
Hepatocitos
/
Hígado
/
Macaca mulatta
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2004
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
Reino Unido
Institución/País de afiliación:
Fiocruz/BR
/
National Institute for Biological Standards and Control/GB
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