Relacion ARN/ADN como indice de condicion fisiologica del hibrido de la cachama Colossoma macropomum y el morocoto piaractus brachypomus durante el desarrollo embrionario / RNA/DNA ratio as an index of physiological condition of Colossoma macropomum and Piaractus branchypomus (Pisces: Characiformes) during embryonic development
Rev. biol. trop
;
51(supl. 4): 91-96, jun. 2003. tab
Artículo
en Español
| LILACS
| ID: lil-388930
RESUMO
We evaluated RNA/DNA ratio as an index of physiological condition during larval development of a hybrid between the fishes Colossoma macropomum (cachama) and Piaractus brachypomus (morocoto). The samples were obtained by induced reproductive technology and the eggs were maintained in acrylic conical incubator with a continuous waterflow. Embryonic development, from egg fertilization to cell division and hatch out, took 12 hours 20 minutes at 29.5 degrees C, dissolved oxygen contents of 6.0 ppm and pH 7.5. Nucleic acids quantification was determined by fluorometry with ethidium bromide and Hoechst 33258 dyes. We observed significant changes of RNA/DNA ratios during all stages of the embryonic larval development. Therefore, RNA/DNA relation is an useful technique to evaluate physiological condition in short period and could be utilized as nutritional condition and/or instantaneous growth for routine check to verify the health status in early life of cultivated species
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Índice:
LILACS (Américas)
Asunto principal:
ADN
/
ARN
/
Peces
Límite:
Animales
Idioma:
Español
Revista:
Rev. biol. trop
Asunto de la revista:
Biologia
/
Medicina Tropical
Año:
2003
Tipo del documento:
Artículo
País de afiliación:
Venezuela
Institución/País de afiliación:
Instituto Nacional de Investigaciones Agricolas/VE
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