5-Aminolevulinic acid and the hepatic oxidative stress in the early phase of experimental hexachlorobenzene intoxication
RBCF, Rev. bras. ciênc. farm. (Impr.)
;
40(2): 203-208, abr.-jun. 2004. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-391026
RESUMO
This work evaluated the levels of 5-aminolevulinic acid (ALA) in the liver of rats exposed to different doses of HCB (25,50, and 100mg/kg b.w. for 4 weeks) and correlated them with lipid peroxidation parameters. Levels of ALA were determined by highpressure liquid chromatography after derivatization with acetylacetone and formaldehyde, followed by fluorescence detection. The methodology was carefully validated, nonetheless hepatic levels of ALA in all animals treated or not were below the detection limit of the method (2.27mg of ALA/g liver). On the other hand, lipid peroxidation, evaluated as thiobarbituric acid reactants production and chemiluminescence was found significantly increased in the livers of all treated rats, in comparison with control values (p<0.05). These results suggest that the hepatic oxidative stress observed in animals following HCB treatment may not necessarily be associated with increased levels of ALA in the liver. Another possibility is that increased levels of ALA in the liver, even below the detection limit of the method are sufficient to induce hepatic oxidative stress
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Peroxidación de Lípido
/
Estrés Oxidativo
/
Ácido Aminolevulínico
/
Hexaclorobenceno
Idioma:
Inglés
Revista:
RBCF, Rev. bras. ciênc. farm. (Impr.)
Asunto de la revista:
Bioquímica
/
Farmacia
/
Farmacología
Año:
2004
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade de São Paulo/BR
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