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Molecular investigation of tRNA genes integrity and its relation to pathogenicity islands in Shiga toxin-producing Escherichia coli (STEC) strains
Novais, Rogério Carlos; Chaves, Marcela Cassin; Gonzalez, Alice Gonçalves Martins; Andrade, João Ramos Costa.
  • Novais, Rogério Carlos; Universidade do Estado do Rio de Janeiro. Faculdade de Formação de Professores. Departamento de Ciências Biológicas. Rio de Janeiro. BR
  • Chaves, Marcela Cassin; Universidade do Estado do Rio de Janeiro. Faculdade de Formação de Professores. Departamento de Ciências Biológicas. Rio de Janeiro. BR
  • Gonzalez, Alice Gonçalves Martins; Universidade Federal Fluminense. Departamento de Bromatologia. Rio de Janeiro. BR
  • Andrade, João Ramos Costa; Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Microbiologia e Imunologia. Rio de Janeiro. BR
Genet. mol. biol ; 27(4): 589-593, Dec. 2004. ilus, tab
Artículo en Inglés | LILACS | ID: lil-391234
RESUMO
tRNA genes are known target sites for the integration of pathogenicity islands (PAI) and other genetic elements, such as bacteriophages, into bacterial genome. In most STEC (Shiga toxin-producing Escherichia coli), the PAI called LEE (locus of enterocyte effacement) is related to bacterial virulence and is mostly associated to the tRNA genes selC and pheU. In this work, we first investigated the relationship of LEE with tRNA genes selC and pheU in 43 STEC strains. We found that 28 strains (65 percent) had a disrupted selC and/or pheU. Three of these strains (637/1, 650/5 and 654/3) were chosen to be submitted to a RAPD-PCR technique modified by the introduction of specific primers (corresponding to the 5'end of genes selC and pheU) into the reaction, which we called "anchored RAPD-PCR". The PCR fragments obtained were transferred onto membranes, and those fragments which hybridized to selC and pheU probes were isolated. One of these fragments from strain 637/1 was partially sequenced. An 85-nucleotide sequence was found to be similar to the cfxA2 gene that encodes a beta-lactamase and is part of transposon Tn4555, a pathogenicity island originally integrated into the Bacteroides genome.
Asunto(s)
Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: ARN de Transferencia / Escherichia coli Límite: Animales Idioma: Inglés Revista: Genet. mol. biol Asunto de la revista: Genética Año: 2004 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Universidade Federal Fluminense/BR / Universidade do Estado do Rio de Janeiro/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: ARN de Transferencia / Escherichia coli Límite: Animales Idioma: Inglés Revista: Genet. mol. biol Asunto de la revista: Genética Año: 2004 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Universidade Federal Fluminense/BR / Universidade do Estado do Rio de Janeiro/BR