In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
Mem. Inst. Oswaldo Cruz
;
99(7): 697-701, Nov. 2004. ilus, tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-391597
RESUMO
Primary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Enfermedades Cardiovasculares
/
Creatina Quinasa
/
Miocitos Cardíacos
/
Isoenzimas
/
L-Lactato Deshidrogenasa
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2004
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
Francia
Institución/País de afiliación:
Fiocruz/BR
/
Institute de Recherches sur le Cancer de Lille/FR
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