Increased production of tumor necrosis factor-alpha in whole blood cultures from children with primary malnutrition
Braz. j. med. biol. res
;
38(2): 171-183, fev. 2005. tab, graf
Artículo
en Inglés
| LILACS, BVSAM
| ID: lil-393653
RESUMO
Because low tumor necrosis factor-alpha (TNF-alpha) production has been reported in malnourished children, in contrast with high production of TNF-alpha in experimental protein-energy malnutrition, we reevaluated the production of TNF-alpha in whole blood cultures from children with primary malnutrition free from infection, and in healthy sex- and age-matched controls. Mononuclear cells in blood diluted 15 in endotoxin-free medium released TNF-alpha for 24 h. Spontaneously released TNF-alpha levels (mean ± SEM), as measured by enzyme immunoassay in the supernatants of unstimulated 24-h cultures, were 10,941 ± 2,591 pg/ml in children with malnutrition (N = 11) and 533 ± 267 pg/ml in controls (N = 18) (P < 0.0001). TNF-alpha production was increased by stimulation with lipopolysaccharide (LPS), with maximal production of 67,341 ± 16,580 pg/ml TNF-alpha in malnourished children and 25,198 ± 2,493 pg/ml in controls (P = 0.002). In control subjects, LPS dose-dependently induced TNF-alpha production, with maximal responses obtained at 2000 ng/ml. In contrast, malnourished patients produced significantly more TNF-alpha with 0.02-200 ng/ml LPS, responded maximally at a 10-fold lower LPS concentration (200 ng/ml), and presented high-dose inhibition at 2000 ng/ml. TNF-alpha production a) was significantly influenced by LPS concentration in control subjects, but not in malnourished children, who responded strongly to very low LPS concentrations, and b) presented a significant, negative correlation (r = -0.703, P = 0.023) between spontaneous release and the LPS concentration that elicited maximal responses in malnourished patients. These findings indicate that malnourished children are not deficient in TNF-alpha production, and suggest that their cells are primed for increased TNF-alpha production.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Células Sanguíneas
/
Leucocitos Mononucleares
/
Trastornos de la Nutrición del Niño
/
Lipopolisacáridos
/
Factor de Necrosis Tumoral alfa
Tipo de estudio:
Estudio observacional
Límite:
Niño
/
Child, preschool
/
Femenino
/
Humanos
/
Lactante
/
Masculino
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
2005
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
Francia
Institución/País de afiliación:
Département de l'Évaluation Scientifique de l'INSERM/FR
/
FIOCRUZ/BR
/
Institut Pasteur de Paris/FR
/
Universidade Federal do Rio de Janeiro/BR
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