A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics
Braz. j. med. biol. res
; 38(3): 445-451, mar. 2005. ilus
Article
en En
| LILACS
| ID: lil-394797
Biblioteca responsable:
BR1.1
ABSTRACT
It is widely accepted that the classical constant-temperature hot-plate test is insensitive to cyclooxygenase inhibitors. In the current study, we developed a variant of the hot-plate test procedure (modified hot-plate (MHP) test) to measure inflammatory nociception in freely moving rats and mice. Following left and right hind paw stimulation with a phlogogen and vehicle, respectively, the animals were placed individually on a hot-plate surface at 51°C and the withdrawal latency for each paw was determined simultaneously in measurements performed at 15, 60, 180, and 360 min post-challenge. Plantar stimulation of rats (250 and 500 æg/paw) and mice (125-500 æg/paw) with carrageenan led to a rapid hyperalgesic response of the ipsilateral paw that reached a plateau from 15 to 360 min after challenge. Pretreatment with indomethacin (4 mg/kg, ip) inhibited the phenomenon at all the times analyzed. Similarly, plantar stimulation of rats and mice with prostaglandin E2 (0.5 and 1 æg/paw) also resulted in rapid hyperalgesia which was first detected 15 min post-challenge. Finally, we observed that the MHP test was more sensitive than the classical Hargreaves' test, being able to detect about 4- and 10-fold lower doses of prostaglandin E2 and carrageenan, respectively. In conclusion, the MHP test is a simple and sensitive method for detecting peripheral hyperalgesia and analgesia in rats and mice. This test represents a low-cost alternative for the study of inflammatory pain in freely moving animals.
Texto completo:
1
Índice:
LILACS
Asunto principal:
Dimensión del Dolor
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Antiinflamatorios no Esteroideos
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Indometacina
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Calor
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Hiperalgesia
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
Asunto de la revista:
BIOLOGIA
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MEDICINA
Año:
2005
Tipo del documento:
Article
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Project document