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Feasibility of refreezing human spermatozoa through the technique of liquid nitrogen vapor
Verza Júnior, Sidney; Esteves, Sandro C.
  • Verza Júnior, Sidney; Center for Male Infertility, ANDROFERT. Campinas. BR
  • Esteves, Sandro C; Center for Male Infertility, ANDROFERT. Campinas. BR
Int. braz. j. urol ; 30(6): 487-493, Nov.-Dec. 2004. tab
Artículo en Inglés | LILACS | ID: lil-397810
RESUMO

OBJECTIVE:

To assess the feasibility of refreezing human semen using the technique of liquid nitrogen vapor with static phases. MATERIALS AND

METHODS:

Twenty samples from 16 subjects who required disposal of their cryopreserved semen were thawed, corresponding to 6 cancer patients and 10 participants in the assisted reproduction (AR) program. Samples were refrozen using the technique of liquid nitrogen vapor with static phases, identical to the one used for the initial freezing, and thawed again after 72 hours. We assessed the concentration of motile spermatozoa, total and progressive percent motility and spermatic vitality, according to criteria of the World Health Organization (WHO), as well as spermatic morphology according to the strict Kruger criterion, after the first and after the second thawing.

RESULTS:

We observed a significant decrease in all the parameters evaluated between the first and the second thawing. Median values for the concentration of motile spermatozoa decreased from 2.0x10(6)/mL to 0.1x10(6)/mL (p < 0.01); total percent motility from 42 percent to 22.5 percent (p < 0.01); progressive percent motility from 34 percent to 9.5 percent (p < 0.01); vitality from 45 percent to 20 percent (p < 0.01); and morphology from 5 percent to 5 percent (p = 0.03). There was no significant difference in the spermatic parameters between the cancer and assisted reproduction groups, both after the first and after the second thawing. We observed that in 100 percent of cases there was retrieval of motile spermatozoa after the second thawing.

CONCLUSIONS:

Refreezing of human semen by the technique of liquid nitrogen vapor allows the retrieval of viable spermatozoa after thawing.
Asunto(s)
Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Preservación de Semen / Espermatozoides / Criopreservación Límite: Adulto / Humanos / Masculino Idioma: Inglés Revista: Int. braz. j. urol Asunto de la revista: Urología Año: 2004 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Center for Male Infertility, ANDROFERT/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Preservación de Semen / Espermatozoides / Criopreservación Límite: Adulto / Humanos / Masculino Idioma: Inglés Revista: Int. braz. j. urol Asunto de la revista: Urología Año: 2004 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Center for Male Infertility, ANDROFERT/BR